LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

Abstract Background Cervical cancer (CC) is one of the most common gynaecological malignancies all around world. The mechanisms cervical carcinoma formation remain under close scrutiny. long non-coding RNAs (lncRNA) and microRNAs (miRNAs) play important roles in controlling gene expression promoting development progression by acting as competitive endogenous RNA (ceRNA). However, lncRNA associated with ceRNAs carcinogenesis remains unknown. In this study, HOTAIR was investigated HPV16 positive cells, candidate miRNAs target genes were identified to clarify putative HOTAIR/miRNA cells. Methods proliferation ability cells measured CCK8 EdU incorporation assays cell apoptosis analyzed flow cytometry. HOTAIR, miR-214-3p, E7 mRNA detected qRT-PCR. As for searching interaction between miR-214-3p binding sites on predicted starbase v2.0 database, then dual-luciferase assay used verify sites. addition, Gene Ontology (GO) protein–protein (PPI) network analysis performed bioinformatics analysis. potential signal pathway regulated HOTAIR/miR-214-3p KEGG enrichment confirmed qPCR WB Results Our results showed that up-regulated, while down-regulated HPV16-positive status played an role regulating or knockdown could significantly inhibited proliferate promote cellular apoptosis, whereas inhibition partially reversed such results. Bioinformatics 1451 miR-214-3p. ontology Pathway these mainly related regulation communication, protein binding, enzyme transferase activity, Wnt ligand biogenesis. enriched Wnt/β-catenin signaling pathway. Finally, our inhibit β-catenin Conclusion act a ceRNA through cancer. HOTAIR/miR-214-3p/Wnt/β-catenin might provided new insight into defining novel biomarkers